ABSTRACT
Various kinds of field crops growing on two commercial farms in the Whitehorse area of the southern Yukon Territory were surveyed for diseases in summer 2020 by staff of the Agriculture Branch of the Government of Yukon. They included barley, wheat, canola, beets, broccoli, cabbage, carrots, potatoes and turnips. Fields were visited one or more times during July and August. The incidence and severity of diseases were visually assessed on a crop-by-crop basis and samples were collected for laboratory analysis of the pathogens present, if any. Both infectious and non-infectious diseases were present on most crops. The infectious diseases were caused by various species of plant pathogenic bacteria and fungi that were common on these crops growing in other areas of Canada. INTRODUCTION AND METHODS: The 2020 field crop disease survey is believed to be the first organized study of its kind on agricultural crops in the Territory. In his book, "An Annotated Index of Plant Diseases in Canada . . . ", I.L. Conners lists over 300 records of plant diseases on trees, shrubs, herbs and grasses in the Yukon that were published by individuals who were surveying forests and native vegetation mainly for federal government departments, universities and other agencies (Conners 1967). The objectives of the 2020 survey were: (1) to determine the kinds and levels of diseases on selected Yukon crops, (2) to identify the major pathogen species attacking Yukon crops, and (3) to use the results to plan future surveillance activities aimed at helping producers to improve their current disease management programs. All of the fields included in the 2020 survey were situated on two commercial farms, which were designated as Farm #1 and #2, in the Whitehorse area in the southern Yukon (Fig. 1). The crops surveyed included cereals (barley and wheat), oilseeds (canola) and vegetables (beets, broccoli, cabbage, carrots, potatoes and turnips). Fields were visited one or more times in the mid- to late growing season (July/August) at a time when damage from diseases was most noticeable. Symptoms were visually assessed on a crop-by-crop basis by determining their incidence and severity. Incidence was represented by the percentage of plants, leaves, heads, kernels, etc., damaged in the target crop, while severity was estimated to be the proportion of the leaf, fruit, head, root/canopy area, etc., affected by a specific disease as follows: Proportion of the canopy affected based on a 0-4 rating scale, where: 0 = no disease symptoms, 1 = 1-10% of the crop canopy showing symptoms;2 = 11-25% showing symptoms, 3 = 26- 50% showing symptoms, and 4 = > 50% showing symptoms. Photographs of affected plants were taken and sent to plant pathologists across Western Canada for their opinions on causation. Where possible, representative samples of plants with disease symptoms were packaged and sent to the Alberta Plant Health Lab (APHL) in Edmonton, AB for diagnostic analyses. Background information, such as the general cultural practices and cropping history, was obtained from the producers wherever possible. GPS coordinates were obtained for each field to enable future mapping Cereals: Individual fields of barley (11 ha) and wheat (30 ha) located at Farm #1 were surveyed. The barley was a two-row forage cultivar 'CDC Maverick', while the wheat was an unspecified cultivar of Canada Prairie Spring (CPS) Wheat. Plant samples were taken along a W-shaped transect for a total of five sampling points for the barley field (< 20 ha) and ten sampling points for the wheat field (> 20 ha). The first visit, which occurred on July 30, involved visual inspection and destructive sampling wherein plants were collected and removed from the field for a detailed disease assessment at a lab space in Whitehorse. There, the roots were rinsed off and the plants were examined for disease symptoms. The second visit to these fields, which occurred on August 27, only involved visual examination of the standing crop. Oilseeds: A single 40 ha field of Polish canola (cv. 'Synergy') was examined o
ABSTRACT
In this study, we studied the biochemical characterization of flavone synthase I from Daucus carota (DcFNS I) and applied it with flavonoid 6-hydroxylase from Scutellaria baicalensis (SbCYP) to convert flavanones to flavones. The recombinant DcFNS I was expressed in the form of the glutathione-S-transferase fusion protein. Rather than taxifolin, naringenin, pinocembrin, and eriodictyol were accepted as substrates. The optimal temperature and pH for reaction in vitro were 35 °C and 7.5, respectively, and 2-oxoglutarate was essential in the assay system. Co2+, Cu2+, Mn2+, Ni2+, and Zn2+ were not substitutes for Fe2+. EDTA and pyruvic acid inhibited the activity, except for Fe3+. Kinetic analysis revealed that the Vmax and kcat values of the recombinant DcFNS I against naringenin were 0.183 nmol mg-1 s-1 and 0.0121 s-1, and 0.175 nmol mg-1 s-1 and 0.0116 s-1 against pinocembrin. However, the recombinant DcFNS I had a higher affinity for naringenin than pinocembrin, with kM values for each of 0.076 mM and 0.174 mM respectively. Thus, it catalyzed naringenin more efficiently than pinocembrin. Subsequently, using an Escherichia coli and Saccharomyces cerevisiae co-culture system, we successfully converted naringenin and pinocembrin to scutellarein and baicalein respectively. In a synthetic complete medium, the titers of scutellarein and baicalein reached 5.63 mg/L and 0.78 mg/L from 200 mg/L precursors.
ABSTRACT
Edible vaccines are created from transgenic plants and animals and contain immunostimulant. Edible vaccines, to put it simply, are medications generated from plants or animals. In underdeveloped countries, oral vaccines are less expensive and more widely available. Researchers came up with the idea of edible vaccines, in which edible plant pieces are employed as a vaccine factory. To make edible vaccinations, scientists put desired genes into plants and then force the plants to generate the proteins expressed in the genes. Transgenic plants are the result of transformation, whereas transformation is the act of converting plants. The edible vaccination promotes mucosal immunity. Dendritic cells in the gut can assist native T cells activate and differentiate into follicular T-helpers (Tfh). T and B cells will respond precisely to a reliable, digestible immunization. Potato, tomato, banana, carrots, tobacco, papaya, algae, and a variety of other plants are utilised as alternative agents for standard vaccinations. Malaria, cholera, hepatitis, rabies, measles, rotavirus, diarrhoea cancer treatments and treatment of covid-19 are among the illnesses for which plant-based vaccines have been created. It takes time and dedication to develop and sell edible vaccinations. Many edible vaccines for animal and human ailments have been developed and have gone through various levels of clinical testing. The importance of plant-based vaccinations is emphasized in this article.
ABSTRACT
The COVID-19 pandemic presented many challenges for the LSU AgCenter 4-H program. To meet social distancing guidelines and ensure the safety of youth and agents, many of programming efforts transitioned to a virtual format. However, the need remained for hands-on learning opportunities. 4-H programming is driven by the motto "learn by doing", and it was paramount that agents provide purposeful and engaging activities. The 4-H Seed-of-the-Month Club is a project club that emerged to meet that need. Agents created a nine-month seed subscription made available to youth across the region beginning in August 2020. With this project, participating youth were provided seeds of various plants, including pine seedlings, rainbow carrots, easter egg radishes, lettuce, green beans, okra, squash and coneflowers. Agents used the LSU AgCenter Louisiana planting guide to select the seeds to offer each month. In the future, agents and youth alike have expressed an interest in continuing to provide horticulture opportunities through the delivery mode of the Seed-of-the-Month Club. Participants were asked to provide top choices for their gardens by receiving carrots, lettuce and green bean seeds for the future.
ABSTRACT
Covid-19, which started in China and affected all countries in the world in a short time, spread rapidly among people and caused serious loss of life. This global epidemic has not only been effective on human health, but has also brought with it a new world order in economic and social terms. In Turkey, the impact of the epidemic reached critical dimensions in time, especially in the health sector, as well as in the agriculture and industry sectors. The aim of this research is to comparatively evaluate consumer preferences for agricultural and food products before and during Covid-19. The material of the research consists of data obtained from 384 consumers residing in the urban area of Samsun. Research results in the epidemic process;expiry date, price, brand, It shows that the ranking of the most important criteria related to agricultural and food products such as production date and company name has not changed compared to the pre-epidemic period, but the importance of the criteria has increased. According to the results of the research, the highest consumption increase was in garlic with 57.1% compared to the pre-epidemic period, followed by bulgur (45.8%) and carrots (42.3%). It was determined that the most effective factors in purchasing agricultural and food products during the epidemic were price reductions (49.7%) and experience with the purchased product (28.1%). The fact that price reductions are taken into account when purchasing agricultural and food products shows that economic concerns are still a determining criterion for consumers during the epidemic process. however, it shows that the importance of the criteria has increased. According to the results of the research, the highest consumption increase was in garlic with 57.1% compared to the pre-epidemic period, followed by bulgur (45.8%) and carrots (42.3%). It was determined that the most effective factors in purchasing agricultural and food products during the epidemic were price reductions (49.7%) and experience with the purchased product (28.1%). The fact that price reductions are taken into account when purchasing agricultural and food products shows that economic concerns are still a determining criterion for consumers during the epidemic process. however, it shows that the importance of the criteria has increased. According to the results of the research, the highest consumption increase was in garlic with 57.1% compared to the pre-epidemic period, followed by bulgur (45.8%) and carrots (42.3%). It was determined that the most effective factors in purchasing agricultural and food products during the epidemic were price reductions (49.7%) and experience with the purchased product (28.1%). The fact that price reductions are taken into account when purchasing agricultural and food products shows that economic concerns are still a determining criterion for consumers during the epidemic process. It was determined that the most effective factors in purchasing agricultural and food products during the epidemic were price reductions (49.7%) and experience with the purchased product (28.1%). The fact that price reductions are taken into account when purchasing agricultural and food products shows that economic concerns are still a determining criterion for consumers during the epidemic process. It was determined that the most effective factors in purchasing agricultural and food products during the epidemic were price reductions (49.7%) and experience with the purchased product (28.1%). The fact that price reductions are taken into account when purchasing agricultural and food products shows that economic concerns are still a determining criterion for consumers during the epidemic process.